About event
At the molecular level, adaptive advantages to environmental changes are primarily provided by epigenetic machinery, including histone proteoforms. The scope of knowledge in the field of histone epigenetics is determined by the feasibility of respective analyses, which are challenging due to the complex pattern of histone post-translational modifications (PTMs) and the presence of histone variants. In this respect, proteomics offers unrivaled opportunities for in-depth investigation of histone function in the regulation of gene expression and cell fate.
This talk will uncover:
- The complexity of histone proteoforms. Multiple histone variants, their modification diversity, and histone epigenetic dynamics will be discussed.
- Method development for histone analysis.
- The development of sample preparation methods for improved detection of modified histone forms by LC-MS/MS will be shown, with a particular focus on the improvement of chromatographic properties of histone peptides using novel derivatization agents.
- Disease formation due to the imbalance in histone modification status. The advantage of proteomic analyses in revealing specific changes in histone proteoforms and other proteins in a biological context will be demonstrated using an example study aimed at male fertility disorders.
More information
This event is part of the Principal Investigator Seminar Series, the schedule of seminars can be found here.
