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We developed a method for absolute quantification of miRNA by its expression along with the expression of endogenous control gene. It is based on the use of synthetic standards for miRNA and endogenous control, which provides absolute number of miRNA copies, and precise cut-offs.
This technology can be used for:
To quantify miRNAs as biomarkers.
Equipment
- qRT-PCR
- NanoDrop
- 2100 Bioanalyzer
