About event
This course is for students, postdocs, and staff who want to learn the fundamentals of light microscopy. Through a combination of theory and hands-on practice, you will explore image formation, optical resolution limits, key microscope components, and much more.
You will gain practical experience in acquiring images using various microscopy techniques, including transmitted light microscopy, differential interference contrast (DIC), phase contrast, fluorescence widefield and confocal imaging.
The final part of the course focuses on image processing and analysis, teaching you how to properly process and analyze images using specialized software.
Registration fees
- Academic: 1 200 CZK + VAT
- Commercial: 3 300 CZK + VAT
Programme
Tuesday, June 24
Theoretical part - principles of light microscopy, transmitted light microscopy, different contrasting techniques - DIC, dark field, phase contrast, fluorescence, widefield microscopy, optical sectioning methods - apotome, confocal microscopy
Wednesday, June 25
Practical part - image acquisition using different microscopy platforms
- Zeiss AxioImager - transmitted light, BF, DIC, phase contrast, fluorescence, Apotome
- Zeiss AxioZoom - transmitted light imaging, fluorescence, Apotome
- Zeiss LSM880 - laser scanning confocal microscope
- Evident Yokogawa W1 - spinning disc confocal microscope
Thursday, June 26
Practical part - image acquisition using different microscopy platforms
Fundamentals of Image processing and quantification
Practical session Image processing and visualisation
ZEN, FIJI, IMARIS, Cell Profiler
