Analytical ultracentrifugation (AUC ProteomeLab)
Guarantor:
Monika Kubíčková, Ph.D.
Technology / Methodology:
Interakce a charakterizace biomolekul
Instrument status:
Operational, 27.10.2022 09:42
Equipment placement:
Masarykova univerzita, UKB
Research group:
Biomolecular Interactions and Crystallography Core Facility
Description:
Beckman Coulter ProteomeLab XL-I analytical ultracentrifuge is equipped with absorbance (wavelength range 190 – 800 nm) and interference optics and can be used for both
sedimentation velocity and sedimentation equilibrium experiments. Analytical ultracentrifugation is applicable for proteins, nucleic acids, lipids, polysaccharides, viruses and nanoparticles. It has a broad applicability including determination of sample
homogeneity, oligomeric state of proteins (or molecular weight, respectively) and can be used
to assess aggregation of sample and to study biomolecular interactions of self- and hetero-association systems (determination of stoichiometry and affinity).