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Professor Radim Chmelik studied physics at Masaryk University Brno and 1997 received his PhD in Physical and Materials Engineering from Brno University of Technology (Czech Republic) under Professor Jiri Komrska for the theoretical description of focusing by diffractive lenses. As a postdoc, he studied methods of 3D imaging in light microscopy. This led him to incoherent-light holography approaches. In the late ninetieth, he and Professor Zdenek Harna built the first holographic microscope capable of working with completely incoherent illumination. He then continued to investigate these techniques as modalities of Quantitative Phase Imaging (QPI) and (since 2007) also their applications in cell biology together with Dr. Pavel Vesely. In 2012, Radim Chmelik became the head of the Experimental Biophotonics research group at CEITEC, Brno. Within the field of advanced light microscope, his group focuses on the progress of Holographic Incoherent-light-source QPI (hiQPI) both in the development and applications. Coherence effects are studied to develop hiQPI of 3D specimens and improve imaging in turbid media. Novel techniques and image processing methods are applied for non-invasive analysis of live-cell behavior. Based on live-cell dry-mass profiling, the responses to therapeutic challenges constitute the strategy for developing personalized cancer treatment. In 2016, Radim Chmelík received the most prestigious Czech award, Czech Brains, in the Invention category, and 2022, the Award of the Czechoslovak Microscopy Society for his significant contribution to the development of microscopic methods.