Molecular and Functional Analysis of Casein Kinase 1 Biology

Project type: GACR EXPRO
Project coordinator: Faculty of Science MU
Project partners: CEITEC MU
Grant provider/investor: Czech Science Foundation
Project code: 19-28347X
Implementation period: 01. 01. 2019 – 31. 12. 2023
Total budget: 1 872 461 EUR
Budget for CEITEC MU: 834 409 EUR

Project description:

The aim of this project is to fill several major gaps in our understanding of casein kinase 1 (CK1) mediated cellular functions. CK1 acts as a component of several developmental pathways such as Wnt, Hedgehog or Yap/Taz that are important drivers of tumorigenesis. In this grant proposal we want to understand how CK1 gets activated and mediates the Wnt signalling pathway, a key signalling machinery dependent on CK1. Further, we will identify unique protein targets of individual CK1 isoforms in developmental signalling pathways, and analyse the biological consequences of the selective inhibition of CK1 isoforms in the context of chronic lymphocytic leukemia (CLL), a disease with the proven sensitivity to CK1 inhibition. We will take advantage of the state-of-the-art integrated structural biology, RNA Sequencing and global proteomic analyses in combination with standard cell physiology techniques. This work will provide mechanistic insight into the role of CK1 isoforms and will pave the avenue for the use of isoform-specific CK1 inhibitors in the treatment of cancer.

Principal investigator:

prof. Mgr. Vítězslav Bryja, Ph.D.

Investigators at CEITEC MU: