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TRAP (Telomere Repeat Amplification Protocol) assay is based on initial extension of non-telomeric primer by telomerase activity with telomeric repeats, and subsequent amplification of the elongated product with PCR. It can be performed as a semi-quantitative or a quantitative version.
This technology can be used for:
Potential anti-cancer drug testing – telomerase activity, expression and telomere stability can be used as indicators of efficiency of potential anti-cancer drugs which inhibit telomerase action by diverse means.
Equipment
- Thermocyclers and real-time thermocyclers
- Horizontal and vertical electrophoreses, documentation system
- Centrifuges
